Figure 2From: Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cellsCharacterizing in vitro neural stem cells to study Methyl CpG binding protein 2 (MeCP2) expression. (A) Schematic representation of in vitro neural stem cell (NSC) differentiation. (B) Detection of (a) NESTIN+ and (b) KI67+ cells in self-renewing neurospheres. Scale bars represent 20 μm. (C) Immunofluorescent detection of different cell-type markers in the day 8 (D8) population (a) TUBULIN III (TUB III): neurons, (b) Glial fibrillary acidic protein (GFAP): astrocytes, (c) S100B: mature astrocytes, (d) 2',3'-Cyclic-nucleotide 3'-phosphodiesterase (CNPase): oligodendrocytes, (e) Myelin basic protein (MBP): oligodendrocytes, (f) Oligodendrocyte lineage transcription factor 2 (OLIG2): early oligodendrocytes and progenitors, and (g) KI67: proliferating cells. Scale bars represent 10 μm. The percentages represent average number of cells from three individual experiments (n = 3 ± standard error of the mean). (D) (a) Immunofluorescent detection of MeCP2 in a sectioned primary neurosphere. Scale bar represents 20 μm. (b) Double labeling of MeCP2 and NESTIN within primary neurosphere cells. Scale bar represents 5 μm. (E) Immunofluorescent detection of MeCP2 in D8 cell types: (a) TUB III, (b) GFAP, (c) S100B, (d) CNPase, (e) MBP, (f) OLIG2, and (g) KI67. Scale bars represent 2 μm.Back to article page