Skip to main content

Table 1 Fold-change in RORA promoter-driven luciferase activity in response to DHT or E2

From: Differential recruitment of coregulators to the RORA promoter adds another layer of complexity to gene (dys) regulation by sex hormones in autism

Region of RORA promoter fused to luciferase (bp from TSS)

AR and ER binding sites in promoter region

Fold-change*with DHT (P value¥)

Fold-change*with E2 (P value¥)

−48 to −10055

ARbs-I-III; ERbs-I-IV

0.78 (0.020)

2.29 (0.021)

−48 to −6000

ARbs-I-III; ERbs-II-IV

0.81 (0.008)

0.71 (0.001)

−48 to −2344

ARbs-II-III; ERbs-II-IV

1.22 (0.024)

0.86 (0.035)

−48 to −1992

ARbs-III; ERbs-IV

0.83 (0.007)

0.74 (0.001)

  1. *Fold-change relative to vehicle treatment; ¥two-tailed t test. DHT, 4,5α-dihydrotestosterone; E2, 17β-estradiol; AR, androgen receptor; ERα, estrogen receptor alpha; ARbs, AR potential binding site; ERbs, ER potential binding site.
  2. SH-SY5Y cells were transfected with expression vectors containing different RORA promoter constructs fused to the firefly luciferase gene together with a second expression vector containing the Renilla luciferase gene, at a ratio of 50:1. After a 2-hour treatment with the indicated hormone or vehicle (ethanol), firefly luciferase luminescence was determined for each sample and normalized by Renilla luminescence in the same sample. T tests were performed to determine the significance of the differences between hormone-treated and ethanol-treated samples. There were no significant differences in firefly luciferase activity between hormone-treated and vehicle-treated samples when the cells were transfected with the empty vector (that is, no RORA promoter).