Skip to main content
Figure 5 | Molecular Autism

Figure 5

From: Differential recruitment of coregulators to the RORA promoter adds another layer of complexity to gene (dys) regulation by sex hormones in autism

Figure 5

siRNA-mediated knockdown of SUMO1 and NCOA5 show functional involvement of these coregulators in the regulation of RORA expression by AR and ERα, respectively. (A) SH-SY5Y cells were transfected with siSUMO1 or vehicle control for 24 hours and treated with 1 nM DHT or ethanol for 2 hours. RORA expression was then measured by qRT-PCR analysis (n = 3). Relative RORA quantity in each sample was calculated using a standard curve obtained from 18S expression levels in 10-fold serial dilutions of that sample. ( B ) NCOA5 knockdown was performed in the same manner as for SUMO1 and cells were treated with 1 nM E2. Error bars indicate SEM. Statistical significance of the differences between groups was determined by ANOVA (P <0.001 for each knockdown experiment) followed by post hoc t tests. **P <0.01. AR, androgen receptor; DHT, 4,5α-dihydrotestosterone; E2, 17β-estradiol; ERα, estrogen receptor alpha; SEM, standard error of the mean.

Back to article page

Molecular Autism

ISSN: 2040-2392