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Figure 1 | Molecular Autism

Figure 1

From: Differential recruitment of coregulators to the RORA promoter adds another layer of complexity to gene (dys) regulation by sex hormones in autism

Figure 1

siRNA-mediated knockdown of AR and ERα reveals direct role of hormone receptors in regulation of RORA expression by sex hormones. SH-SY5Y cells were transfected with siAR (A), siERα (B), or vehicle control (mock-transfected) for 24 hours, then treated with 1 nM DHT (A), 1 nM E2 (B), or ethanol for 2 hours. Quantitative RT-PCR analysis (n = 3) of the hormone-treated, transfected cells was conducted to determine RORA expression. Relative RORA quantity in each sample was calculated using a standard curve obtained from 18S expression levels in 10-fold serial dilutions of that sample. Error bars indicate SEM. Statistical significance of the differences between groups was determined by ANOVA (P <0.01 for each knockdown experiment) followed by post hoc t tests. *P <0.05. AR, androgen receptor; DHT, 4,5α-dihydrotestosterone; E2, 17β-estradiol; ERα, estrogen receptor alpha; SEM, standard error of the mean.

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Molecular Autism

ISSN: 2040-2392