Figure 1

mGluR5 receptor density and expression in prefrontal cortex of FXS individuals and healthy controls. (A) Binding curves from homologous competition binding of 0.48 nM of [³H]MPEPy to membrane preparation from FXS and control subject samples at concentrations of unlabeled MPEPy ranging from 0.1 nM to 1 μM. Individual binding curves were obtained from the average of triplicate measurements for each unlabeled ligand concentration. Data represent mean ± standard error in the mean from 14 FXS patients or carriers (FX) and 17 healthy controls (HC). At low concentration of unlabeled ligand, specific binding was higher for FXS than control samples. (B) Results of unpaired t-test to compare the two groups. mGluR5 density tended to be higher (+16%; P = 0.058) in FXS patients than in the control group. Data represent mean ± standard deviation. Solid triangles (▲) in the FX group indicate the location of three FXS carriers; semisolid triangles () indicate the location of a carrier with FXTAS. (C) Representative immunoblot for mGluR5. The mGluR5 band intensity was stronger for the FXS than control subject. Total protein stain of the same lanes confirmed equal-protein loading. (D) Average mGluR5: total protein ratio normalized to control subjects. The ratio was high and marginally significant (+32%; P = 0.048) for the FXS group compared with controls. Data represent mean ± standard deviation. Solid triangles (▲) in the FX group indicate the location of three FXS carriers; semisolid triangles () indicate the location of a carrier with FXTAS.