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Table 6 Quantitative RT-PCR analysis of post-mortem brain tissues from individuals with autism

From: Genome-wide identification of transcriptional targets of RORA reveals direct regulation of multiple genes associated with autism spectrum disorder

Sample comparison (A vs. C) Age (A, C) RORA CYP19A1 ITPR1 A2BP1
Control Autistic A/C Control Autistic A/C Control Autistic A/C Control Autistic A/C
5144A vs. 5251C 20, 19 6.4 2.0 0.316 1.7E-04 5.2E-05 0.307 127.7 70.8 0.554 553.1 105.3 0.190
5173A vs. 5873C 30, 28 38.4 25.4 0.661 1.2E-02 5.9E-03 0.487 246.4 27.3 0.111 830.1 74.2 0.089
6337A vs. 5718C 22, 22 7.9 0.4 0.046 7.7E-06 1.0E-07 0.013 217.4 176.3 0.811 246.0 64.6 0.263
Average ratio (A/C) 17.6 9.3 0.527 4.1E-03 2.0E-03 0.484 197.2 91.5 0.464 543.1 81.4 0.150
P value (unpaired t test)    0.562    0.660    0.137    0.052
   HSD17B10 NLGN1 NTRK2    
Control Autistic A/C Control Autistic A/C Control Autistic A/C    
5144A vs. 5251C 20, 19 128.7 15.7 0.122 178.3 23.8 0.133 3218.9 544.6 0.169    
5173A vs. 5873C 30, 28 126.3 41.1 0.325 255.3 44.9 0.176 2600.1 1077.2 0.414    
6337A vs. 5718C 22, 22 47.6 39.2 0.824 84.8 61.5 0.725 929.8 1265.1 1.361    
Average ratio (A/C) 100.9 32.0 0.317 172.8 43.4 0.251 2249.6 962.3 0.428    
P value (unpaired t test)    0.069    0.062    0.147    
  1. Gene expression in brain tissues from individuals with autism (indicated by ’A’) was compared with that in controls (indicated by ’C’). The numbers preceding ‘A’ or ‘C’ identify specific individuals whose brains were donated to the Autism Tissue Program (San Diego, CA, USA). Total RNA was isolated and purified from frozen post-mortem prefrontal cortex tissues from individuals with autism (n = 3) and the controls (n = 3). Quantitative RT-PCR analyses (in triplicate) were performed to measure expression of RORA and that of six transcriptional targets. The transcript levels of each gene were calculated using standard curves obtained from relative 18S expression in 10-fold serial dilutions of each sample. The numbers highlighted in bold-face type indicate down-regulation of gene expression in autism brain tissues relative to that in respective matched controls. The P values are from unpaired t tests of the expression data from the combined cases versus that of the combined controls.