Quantitative RT-PCR analysis of RORA short hairpin RNA-transfected SH-SY5Y cells. SH-SY5Y cells were transfected with RORA1 short hairpin RNA (shRNA) or negative control shRNA for 24 hours and quantitative RT-PCR analysis (n = 3) was performed to determine expression of RORA1 and potential transcriptional targets in the transfected cells. GAPDH was included as a negative control. Error bars indicate standard error of the mean. *P <0.05, **P <0.01.