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Figure 2 | Molecular Autism

Figure 2

From: Genome-wide identification of transcriptional targets of RORA reveals direct regulation of multiple genes associated with autism spectrum disorder

Figure 2

Chromatin immunoprecipitation-quantitative PCR of RORA transcriptional targets. Chromatin immunoprecipitation followed by quantitative PCR analysis was conducted to determine whether RORA protein binds to A2BP1, CYP19A1, HSD17B10, ITPR1, NLGN1, and NTRK2 promoters. Chromatin was isolated from SH-SY5Y cells and immunoprecipitated with anti-RORA1 or IgG antibody. DNA was purified from the immunoprecipitated chromatin and quantitative real-time PCR analysis (n = 3) was performed to determine the enrichment of each RORA binding element in the promoter region of the selected potential RORA targets. Error bars indicate standard error of the mean. * P <0.05; ** P <0.01; #undetectable.

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