A flowchart showing the study design. One SNP list from each of the four primary analyses in the publication from Anney et al.  (totaling four lists of SNPs) was annotated for eQTL information against an LCL eQTL dataset (http://www.SCANdb.org), parietal tissue and cerebellar tissue. No enrichment was found in any of the LCL annotated datasets, enrichment was found in the parietal lobe for all four analyses and in cerebellar tissue for two of the datasets. The eQTL found in each of the six analyses were combined in order to identify unique eQTL signals that appeared in one or more tissues and in one or more primary analyses. This complete list of unique eQTL signals was then investigated for genes and pathways of interest in autism. eQTL: expression quantitative trait loci; LCL: lymphoblastoid cell line.