Table 1 Antibodies used for histopathological characterization of BTBR mice
Antigen | Vendor | Catalog # | Host | Dilution | Retrieval | IHC Method |
|---|---|---|---|---|---|---|
BrdU | Accurate Chemical (Westbury, NY) | OBT0030 | Rat IgG | 1:300 | None | Free-floating |
| Â | Roche Applied Science (Indianapolis, IN) | 11170376001 | Mouse IgG1 | 1:500 | None | Free-floating |
CNPase | Sigma-Aldrich (St Louis, MO) | C9743 | Rabbit IgG | 1:400 | Borg | Paraffin |
Drebrin | Sigma-Aldrich (St Louis, MO) | D3816 | Rabbit IgG | 1:1300 | None | Paraffin |
Doublecortin | Santa Cruz Biotechnology (Santa Cruz, CA) | sc-8066 | Goat IgG | 1:2000 | None | Free-floating |
GAD65 | Millipore (Temecula, CA) | AB5082 | Rabbit IgG | 1:500 | None | Paraffin |
GAD67 | Millipore (Temecula, CA) | MAB5406 | Mouse IgG2a | 1:1000 1:6000 | Borg | Paraffin Free-floating |
GFAP | Dako (Carpinteria, CA) | Z0334 | Rabbit IgG | 1:5000 | Borg | Paraffin |
| Â | Sigma-Aldrich (St Louis, MO) | G9269 | Rabbit IgG | 1:300 | None | Free-floating |
GluR1 | Millipore (Temecula, CA) | 04-855 | Rabbit IgG | 1:50 | Borg | Paraffin |
Iba1 | Wako Chemicals USA (Richmond, VA) | 19-19741 | Rabbit IgG | 1:1000 | Borg | Paraffin |
MAP2 | Sigma-Aldrich (St Louis, MO) | M4403 | Mouse IgG1 | 1:1000 | Borg | Paraffin |
MBP | Abcam (Cambridge, MA) | ab2404 | Rabbit IgG | 1:50 | Borg | Paraffin |
NeuroD | Santa Cruz Biotechnology (Santa Cruz, CA) | sc-1086 | Goat IgG | 1:250 | None | Free-floating |
NeuN | Millipore (Temecula, CA) | MAB377 | Mouse IgG1 | 1:1000 | None | Free-floating |
NG2 | Millipore (Temecula, CA) | AB5320 | Rabbit IgG | 1:1200 | None | Free-floating |
Parvalbumin | EMD/Calbiochem (Gibbstown, NJ) | PC255L | Rabbit IgG | 1:700 | Borg | Paraffin |
PSA-NCAM | Abcys (Paris, France) | AbC0019 | Mouse IgM | 1:5000 | None | Free-floating |
| Â | Gift from Dr. T Seki | None | Mouse IgM | 1:500 | None | Free-floating |
PSD-95 | Cell Signaling Technology (Beverly, MA) | 3409 | Rabbit IgG | 1:100 | Borg | Paraffin |
Synapsin 2 | Sigma-Aldrich (St Louis, MO) | S2947 | Rabbit IgG | 1:1600 1:30,000 | None | Paraffin Free-floating |
Synaptophysin | Abcam (Cambridge, MA) | 52636 | Rabbit IgG | 1:250 | Borg | Paraffin |
VGluT1 | Synaptic Systems (Goettingen, Germany) | 135303 | Rabbit IgG | 1:900 1:16,000 | None | Paraffin Free-floating |