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Table 1 Antibodies used for histopathological characterization of BTBR mice

From: Histopathologic characterization of the BTBR mouse model of autistic-like behavior reveals selective changes in neurodevelopmental proteins and adult hippocampal neurogenesis

Antigen Vendor Catalog # Host Dilution Retrieval IHC Method
BrdU Accurate Chemical (Westbury, NY) OBT0030 Rat IgG 1:300 None Free-floating
  Roche Applied Science (Indianapolis, IN) 11170376001 Mouse IgG1 1:500 None Free-floating
CNPase Sigma-Aldrich (St Louis, MO) C9743 Rabbit IgG 1:400 Borg Paraffin
Drebrin Sigma-Aldrich (St Louis, MO) D3816 Rabbit IgG 1:1300 None Paraffin
Doublecortin Santa Cruz Biotechnology (Santa Cruz, CA) sc-8066 Goat IgG 1:2000 None Free-floating
GAD65 Millipore (Temecula, CA) AB5082 Rabbit IgG 1:500 None Paraffin
GAD67 Millipore (Temecula, CA) MAB5406 Mouse IgG2a 1:1000 1:6000 Borg Paraffin Free-floating
GFAP Dako (Carpinteria, CA) Z0334 Rabbit IgG 1:5000 Borg Paraffin
  Sigma-Aldrich (St Louis, MO) G9269 Rabbit IgG 1:300 None Free-floating
GluR1 Millipore (Temecula, CA) 04-855 Rabbit IgG 1:50 Borg Paraffin
Iba1 Wako Chemicals USA (Richmond, VA) 19-19741 Rabbit IgG 1:1000 Borg Paraffin
MAP2 Sigma-Aldrich (St Louis, MO) M4403 Mouse IgG1 1:1000 Borg Paraffin
MBP Abcam (Cambridge, MA) ab2404 Rabbit IgG 1:50 Borg Paraffin
NeuroD Santa Cruz Biotechnology (Santa Cruz, CA) sc-1086 Goat IgG 1:250 None Free-floating
NeuN Millipore (Temecula, CA) MAB377 Mouse IgG1 1:1000 None Free-floating
NG2 Millipore (Temecula, CA) AB5320 Rabbit IgG 1:1200 None Free-floating
Parvalbumin EMD/Calbiochem (Gibbstown, NJ) PC255L Rabbit IgG 1:700 Borg Paraffin
PSA-NCAM Abcys (Paris, France) AbC0019 Mouse IgM 1:5000 None Free-floating
  Gift from Dr. T Seki None Mouse IgM 1:500 None Free-floating
PSD-95 Cell Signaling Technology (Beverly, MA) 3409 Rabbit IgG 1:100 Borg Paraffin
Synapsin 2 Sigma-Aldrich (St Louis, MO) S2947 Rabbit IgG 1:1600 1:30,000 None Paraffin Free-floating
Synaptophysin Abcam (Cambridge, MA) 52636 Rabbit IgG 1:250 Borg Paraffin
VGluT1 Synaptic Systems (Goettingen, Germany) 135303 Rabbit IgG 1:900 1:16,000 None Paraffin Free-floating
  1. IHC = immunohistochemistry; BrdU = 5-bromo-2'-deoxyuridine; CNPase = 2',3'-cyclic nucleotide 3'-phosphodiesterase; GAD65 = glutamic acid decarboxylase 65 kDa isform; GAD67 = glutamic acid decarboxylase 67 kDa isoform; GFAP = glial fibrillary acidic protein; GluR1 = glutamate receptor 1; Iba1 = ionized calcium-binding adaptor molecule 1; MAP2 = microtubule-associated protein 2; MBP = myelin basic protein;; NeuN = neuronal nuclei; PSA-NCAM = poly-sialic acid neural cell adhesion molecule; PSD-95 = postsynaptic density protein of 95 kDa; VGluT1 = vesicular glutamate transporter 1