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Table 3 Western blot analysis results for FMRP, mGluR5, GABRβ3, GFAP and β-actin and their ratios in BA9a

From: Dysregulation of fragile X mental retardation protein and metabotropic glutamate receptor 5 in superior frontal cortex of individuals with autism: a postmortem brain study

Children Control Autistic Change P value Cohen's d
FMRP/β-actin 0.073 ± 0.054 0.119 ± 0.062 ↑63% ns 0.76
mGluR5 dimer/β-actin 0.151 ± 0.179 0.405 ± 0.131 ↑168% 0.023b 1.71b
mGluR5 total/β-actin 0.16 ± 0.187 0.466 ± 0.131 ↑191% 0.011b 2.01b
GABRβ3/β-actin 0.085 ± 0.049 0.187 ± 0.128 ↑120% ns 0.96b
GFAP/β-actin 1.6 ± 1.16 3.78 ± 0.962 ↑136% 0.012b 2.09b
β-actin 20.1 ± 2.94 18.3 ± 4.79 ↓8.9% ns -
Adults Control Autistic Change P value Cohen's d
FMRP/β-actin 0.175 ± 0.075 0.087 ± 0.055 ↓50% 0.042b 1.43b
mGluR5 dimer/β-actin 0.136 ± 0.069 0.267 ± 0.175 ↑96% ns 0.87b
mGluR5 total/β-actin 0.139 ± 0.069 0.272 ± 0.178 ↑96% ns 0.87b
GABRβ3/β-actin 0.136 ± 0.069 0.159 ± 0.087 ↑17% ns 0.27
GFAP/β-actin 1.93 ± 0.762 3.04 ± 0.758 ↑58% 0.033b 1.46b
β-actin 23.2 ± 1.97 21.7 ± 3.28 ↓6.5% ns -
  1. aFMRP, fragile X mental retardation protein; mGluR5, metabotropic glutamate receptor 5; GABRβ3, γ-aminobutyric acid (GABA) A receptor β3; GFAP, glial fibrillary acidic protein; BA9, Brodmann's area 9; ns, not significant. bstatistically significant.