Calbindin immunofluorescent staining in the cerebellar cortex. Calbindin immunofluorescent staining of the cerebellar cortex indicates decreased numbers of PCs and diminished complexity of the PC dendritic arborization compared with age-matched controls. Similar changes were observed in all vermal lobules and in lateral posterior lobe cortex. (A) Vermal inferior lobule and (B) lateral posterior lobe cerebellar cortex. Original magnification ×10; scale bar = 250 μm.