Decreased density of GluR1-immunoreactive puncta in Shank3 heterozygous mice. (A and B) High-resolution confocal images of puncta from wild-type and heterozygous mice. (C and D) The same images from Figures 4A and 4B are shown after deconvolution. (E and F) The puncta were quantified after thresholding of fluorescence intensity. (G) Quantification of GluR1-immunoreactive puncta. *P < 0.005. WT, wild-type mice; Het, Shank3 heterozygous mice.