Hypersensitive pupillary light reflex in infants at risk for autism
© Nyström et al.; licensee BioMed Central. 2015
Received: 22 October 2014
Accepted: 16 February 2015
Published: 3 March 2015
Post mortem brain tissue data and animal modeling work indicate cholinergic disruptions in autism. Moreover, the cholinergic system plays a key role in the early neurodevelopmental processes believed to be derailed early in life in individuals with the disorder. Yet, there is no data from human infants supporting a developmentally important role of this neurotransmitter system. Because the pupillary light reflex depends largely on cholinergic synaptic transmission, we assessed this reflex in a sample of infants at risk for autism as well as infants at low (average) risk.
Ten-month-old infants with an older sibling with autism (n = 29, 16 females), and thus a genetic predisposition to developing the disorder themselves, were presented with white flashes on a computer monitor, and pupillary responses were captured using eye tracking. A control group matched on age and developmental level (n = 15, seven females) was also tested.
The siblings of children with autism had a faster and stronger pupillary light reflex compared to control infants. Baseline pupil diameter was equal in the two groups, ruling out tonic autonomic imbalance as an explanation for these differences.
This study establishes that infant siblings of children with autism have hypersensitive pupillary light reflexes, a result which supports the view that altered sensory processing in infancy is associated with elevated autism risk. Moreover, the study indicates that individual differences in autism susceptibility are linked to differences in the cholinergic system during an early developmental period.
Autism spectrum disorder (herein termed autism) is characterized by early emerging social communication and interaction deficits alongside restricted, repetitive patterns of behaviors and interests. It is a heterogeneous and common neurodevelopmental disorder and poses substantial challenges to the individual and the family as well as the wider society. Little is known about the early neurodevelopmental events that shape the autistic behavioral phenotype, which typically emerges during toddlerhood and early childhood . Therefore, many studies are currently being carried out on infants at risk for autism (for example, siblings of children with the disorder, in which the recurrence rate may be as high as 20%) which can inform about processes in infancy that are associated with autism susceptibility [2,3].
Here, we tested the hypothesis that the pupillary light reflex is altered in infants at risk for autism. There were several reasons for this focus. First, the pupillary light reflex regulates the amount of light that reaches the retina, and recent theories of autism as well as the latest edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) view altered reactivity to sensory input as a core defining feature of the disorder [4,5]. Second, although no data on the pupillary light reflex are available from infants, a study of adults with autism found strikingly reduced pupillary light reflex compared to controls . Finally, autism research is in strong need for biological markers that can inform the search for precise molecular mechanisms and that have high translational potential . The pupillary light reflex is noninvasively assessable and is mediated by a well-characterized and relatively simple neural circuit . Specifically, the reflex arc consists of four neurons, bypassing the thalamus and cortex. It depends on synaptic transmission in the Edinger-Westphal nucleus and the ciliary ganglia, both of which are highly acetylcholine-dependent [8,9]. For this reason, the pupillary light reflex has been seen as an index of the integrity of the cholinergic system in other brain disorders . The cholinergic system plays a key role in the pre- and postnatal neurodevelopmental processes believed to be derailed early in life in individuals with autism, including neuronal pathfinding and cell survival [11,12]. Neuroimaging findings as well as post mortem brain tissue data [13-15], rodent models [16,17], and molecular genetic studies  suggest that alterations of the cholinergic system are involved in the etiology of autism. Critically, however, there is no data from infants available to support this hypothesis.
A demonstration that infants at risk for autism have an altered pupillary light reflex would add credibility to the view that individual differences in autism susceptibility early in life are linked to the development of the cholinergic system. In this study, we report the relative constriction of the pupil and the latency to constriction onset in a group of infants at risk and in a control group.
Study group characteristics, final samples (mean/standard deviation)
HR n = 29
LR n = 15
Pairwise comparison ( P value a )
MSELb total score
Data collection and analysis
Computational analysis was done in MATLAB (The MathWorks, Inc., ) using the TimeStudio framework . Gaps in the data series (maximum two samples) were linearly interpolated, and trials with <90% looking time during the time interval 0- to 1,000-ms post flash were discarded. This conservative criterion ensured that eye blinks (which produce >100-ms data loss) were not influencing the pupil measures. Infants with less than four valid trials were excluded from further study (n = 11, see above for details). Pupillary light reflex latencies were calculated according to established standards : a five-point second-order polynomial moving Savitzky-Golay filter was applied before resampling the signal to 300 Hz to achieve better temporal resolution. The velocity was calculated by taking the derivative of the pupil-size signal, and the acceleration was calculated by taking the derivative of the velocity. A centered 25-point moving average filter was applied to the pupil size, velocity, and acceleration traces before further processing. The latency was defined as the absolute acceleration maximum in the interval 100 to 500 ms for each trial, and the median latency across trials was used as the dependent measure in final statistical analyses.
Analyses of the data distributions confirmed the normality and homogeneity of variance. We used statistical tests (independent t-test) with two-tailed probabilities (α = 0.05) unless otherwise stated. Group comparisons were performed using the independent t-test with bootstrapping (10,000 permutations) to obtain robust confidence intervals (running the analyses with conventional t-tests yielded the same pattern as reported below but with lower P values (amplitude P < .001; latency P = .025)).
The relative constriction in response to the light flashes was larger in high-risk (mean 47.84%, standard deviation (SD) 5.84%) than in low-risk (mean 39.89%, SD 8.29%) infants (t(42) = 3.70, P = .003). Moreover, the latency of the reflex onset was significantly shorter in high-risk (mean 261.38 ms, SD 19.27 ms) than in low-risk (mean 276.00 ms, SD 20.94 s) infants (t(42) = 2.32, P = .029). Thus, both measures demonstrated hypersensitive pupillary light reflexes in the high-risk group. Pupil data for the two groups are shown in Figure 2.
There were no differences between the high-risk and low-risk groups in percent looking time on the screen during a 10-s interval before the flash (t(42) = .97, P = .34), showing that the difference in the pupillary response cannot be explained by different saturation of the retina in the two groups. Also, there were no correlations between the percentage looking and the pupillary light reflex measures. Neither the correlation between looking time and relative constriction (r(42) = .09, P = .570, Pearson correlation) nor the correlation between looking time and latency (r(42) = −.02, P = .900) was significant.
Tonic autonomic imbalance would be expected to cause altered pupil diameter at baseline, but not any difference in the latency of the pupillary light reflex [8,9]. Baseline (A0) pupil diameter was similar between the two groups (high-risk, mean 4.80 mm and SD 0.56 mm; low-risk, mean 4.68 mm and SD 0.54; t(42) = 0.66, P = .498). Thus, the current results cannot be explained by tonic autonomic imbalance in the high-risk group.
This study shows that infants at risk for autism have a hypersensitive pupillary light reflex. Thus, at a general level, the study supports theories emphasizing sensory abnormalities as well as the inclusion of sensory hypo- or hyper-reactivity in the diagnostic criteria for autism [4,5]. Moreover, given the link between the pupillary light reflex and the cholinergic system [8-10], the study also provides specific, albeit indirect, support for the view that cholinergic disruptions could be involved in the etiology of the disorder. The detection of these group differences prior to the emergence of the symptoms of autism  adds credibility to the view that cholinergic disruption may have a causal role, as hypothesized earlier based on nondevelopmental human data and animal work [13-16,18].
It is notable that the direction of the group difference is opposite to what could be expected from one study of the pupillary light reflex in adults, which showed hyposensitivity to light in individuals with autism . However, a recent study that included children aged 6 years and older indicated that the magnitude of the difference (autism vs. typical) was smaller (and absent for some measures) in the youngest children . This finding, together with the current data, indicates that chronological age is an important factor when interpreting the pupillary light reflex effect in relation to autism risk. Reversal of group differences as a function of time has been reported by at least two recent studies comparing autistic infants with controls [26,27].
Recent studies indicate that shared environmental influences are negligible in autism, and the genetic risk is predominantly due to common variants ([28,29] but see ). If correct, studies of infant siblings primarily shed light on the early processes associated with genetically mediated autism risk. The recurrence rate in infant siblings of children with autism has been estimated to be around 20% based on US data . A European study of siblings reported that more than 30% received an autism diagnosis at follow-up . In addition, a substantial portion of the siblings are expected to develop other significant sociocommunicative, cognitive, or motor problems or delays. Together, this means that about 50% of siblings of children with autism will eventually have significant autism-related developmental concerns .
It is important to note that without follow-up data, the relation between the data presented here and later autism diagnosis is not known. Rather than predicting autism per se, the pupil response could be a part of the early broader autism endophenotype - shared between both affected and unaffected individuals in the high-risk group. Although identifying such endophenotypes is of lower immediate clinical value, it is important for our understanding of the biological processes associated with susceptibility for disease. It is interesting to note that the latency and the amplitude data were unrelated (Figure 2B), but that both measures differed between groups (Figure 2C,D). Thus, the two could reflect separate underlying processes, which may in turn be differentially related to developmental outcomes.
Given the relative simplicity of the reflex arc, the demonstration of an altered pupillary light reflex in infants at risk for autism could help narrow the search for biological mechanisms related to the disorder . The fact that the reflex can be studied in animals adds to this argument. It is notable that the nicotinic acetylcholine receptor subunit alpha 7 is upregulated in individuals with autism  and mediates fast excitatory synaptic transmission in the pupillary light reflex circuit in animals . Thus, although speculative, one hypothesis arising from the current work is that the fast response in the high-risk group is linked to acetylcholine nicotinic receptors, which are known to modulate other autism-related neurotransmitters as well [11,33]. Future studies should also evaluate whether retinal  or axonal  differences could be involved in shaping the altered pupil response in the infants at risk for autism.
That infants at risk for autism have hypersensitive pupillary light reflexes provides support for the view that early cholinergic disruptions could play a role in the etiology of autism and related disorders of development. In addition, the current study suggests that pupillometry could contribute to noninvasive and quick risk assessment in infants in the future.
Availability of supporting data
Ethics approval and consent to participate
Parents provided written informed consent, and the study was approved by the Regional Ethical Board in Stockholm. The study was conducted in accordance with the standards specified in the 1964 Declaration of Helsinki.
Consent for publication
Written informed consent was obtained from the models for publication of their image in this manuscript (Figure 1). The consent is held by the author and is available for review by the Editor-in-Chief.
Diagnostic and Statistical Manual of Mental Disorders
We thank the EASE team: Dr. Linda E. Campbell, Elodie Cauvet, Therese L Ekberg, Johan Lundin Kleberg, Elisabeth Nilsson Jobs, Emilia Thorup and Eric Zander. We thank the participating families, as well as Maria Axnér and Torkel Carlsson for help during data collection.
This research was supported by the Swedish Research Council in partnership with FAS, FORMAS, and VINNOVA (cross-disciplinary research program concerning the mental health of children and young people; grant number 259-2012-24). The work of PN, TFY, and SB was supported by the ESF COST Action BM1004 “Enhancing the Scientific Study of Early Autism” (ESSEA). TFY was supported by The Bank of Sweden Tercentenary Foundation (P12-0270:1). SB was supported by the Swedish Research Council (No. 523-2009-7054). GG was supported by the European Research Council (StG: CACTUS 312292). The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking under grant agreement n° 115300, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007 - 2013) and EFPIA companies’ in kind contribution.
The EASE team
Linda E. Campbell, Elodie Cauvet, Therese L Ekberg, Johan Lundin Kleberg, Elisabeth Nilsson Jobs, Emilia Thorup, Eric Zander
- Zwaigenbaum L, Bryson S, Rogers T, Roberts W, Brian J, Szatmari P. Behavioral manifestations of autism in the first year of life. Int J Dev Neurosci. 2005;23:143–52.View ArticlePubMedGoogle Scholar
- Jones EJH, Gliga T, Bedford R, Charman T, Johnson MH. Developmental pathways to autism: a review of prospective studies of infants at risk. Neurosci Biobehav Rev. 2014;39:1–33.View ArticlePubMed CentralPubMedGoogle Scholar
- Ozonoff S, Young GS, Carter A, Messinger D, Yirmiya N, Zwaigenbaum L, et al. Recurrence risk for autism spectrum disorders: a baby siblings research consortium study. Pediatrics. 2011;128:E488–95.PubMed CentralPubMedGoogle Scholar
- Markram K, Markram H. The Intense World Theory - a unifying theory of the neurobiology of autism. Front Hum Neurosci. 2010;4:29.View ArticleGoogle Scholar
- AmericanPsychiatricAssociation. Diagnostic and statistical manual of mental disorders (5th ed.). Arlington, VA: American Psychiatric Publishing; 2013.View ArticleGoogle Scholar
- Fan XF, Miles JH, Takahashi N, Yao G. Abnormal transient pupillary light reflex in individuals with autism spectrum disorders. J Autism Dev Disord. 2009;39:1499–508.View ArticlePubMedGoogle Scholar
- Ecker C, Spooren W, Murphy DGM. Translational approaches to the biology of autism: false dawn or a new era? Mol Psychiatry. 2013;18:435–42.View ArticlePubMed CentralPubMedGoogle Scholar
- Loewenfeld I. The pupil: anatomy, physiology, and clinical applications. Oxford: Butterworth-Heinemann; 1999.Google Scholar
- Heller PH, Perry F, Jewett DL, Levine JD. Autonomic components of the human pupillary light reflex. Invest Ophthalmol Vis Sci. 1990;31:156–62.PubMedGoogle Scholar
- Fotiou DF, Stergiou V, Tsiptsios D, Lithari C, Nakou M, Karlovasitou A. Cholinergic deficiency in Alzheimer’s and Parkinson’s disease: evaluation with pupillometry. Int J Psychophysiol. 2009;73:143–9.View ArticlePubMedGoogle Scholar
- Abreu-Villaca Y, Filgueiras CC, Manhaes AC. Developmental aspects of the cholinergic system. Behav Brain Res. 2011;221:367–78.View ArticlePubMedGoogle Scholar
- Courchesne E, Pierce K, Schumann CM, Redcay E, Buckwalter JA, Kennedy DP, et al. Mapping early brain development in autism. Neuron. 2007;56:399.View ArticlePubMedGoogle Scholar
- Perry EK, Lee MLW, Martin-Ruiz CM, Court JA, Volsen SG, Merrit J, et al. Cholinergic activity in autism: abnormalities in the cerebral cortex and basal forebrain. Am J Psychiatr. 2001;158:1058–66.View ArticlePubMedGoogle Scholar
- Lee M, Martin-Ruiz C, Graham A, Court J, Jaros E, Perry R, et al. Nicotinic receptor abnormalities in the cerebellar cortex in autism. Brain. 2002;125:1483–95.View ArticlePubMedGoogle Scholar
- Sokol DK, Dunn DW, Edwards-Brown M, Feinberg J. Hydrogen proton magnetic resonance spectroscopy in autism: preliminary evidence of elevated choline/creatine ratio. J Child Neurol. 2002;17:245–9.View ArticlePubMedGoogle Scholar
- Karvat G, Kimchi T. Acetylcholine elevation relieves cognitive rigidity and social deficiency in a mouse model of autism. Neuropsychopharmacology. 2014;39:831–40.View ArticlePubMed CentralPubMedGoogle Scholar
- McTighe SM, Neal SJ, Lin Q, Hughes ZA, Smith DG. The BTBR mouse model of autism spectrum disorders has learning and attentional impairments and alterations in acetylcholine and kynurenic acid in prefrontal cortex. Plos One. 2013;8:11.View ArticleGoogle Scholar
- Leblond CS, Heinrich J, Delorme R, Proepper C, Betancur C, Huguet G, et al. Genetic and functional analyses of SHANK2 mutations suggest a multiple hit model of autism spectrum disorders. PLoS Genet. 2012;8:17.View ArticleGoogle Scholar
- Mullen EM. Mullen Scales of Early Learning: AGS Edition. MN: AGS: Circle Pines; 1995.Google Scholar
- Falck-Ytter T, Rehnberg E, Bölte S. Lack of visual orienting to biological motion and audiovisual synchrony in 3-year-olds with autism. Plos One. 2013;8:e68816.View ArticlePubMed CentralPubMedGoogle Scholar
- MATLAB R2014a, The MathWorks, Inc., Natick, Massachusetts, United States.
- The TimeStudio Project. [http://timestudioproject.com/]
- Bergamin O, Kardon RH. Latency of the pupil light reflex: sample rate, stimulus intensity, and variation in normal subjects. Invest Ophthalmol Vis Sci. 2003;44:1546–54.View ArticlePubMedGoogle Scholar
- Ozonoff S, Young GS, Belding A, Hill M, Hill A, Hutman T, et al. The broader autism phenotype in infancy: when does it emerge? J Am Acad Child Adolesc Psychiatry. 2014;53:398–407.View ArticlePubMedGoogle Scholar
- Daluwatte C, Miles JH, Christ SE, Beversdorf DQ, Takahashi TN, Yao G. Atypical pupillary light reflex and heart rate variability in children with autism spectrum disorder. JAutism Dev Disord. 2013;43:1910–25.View ArticleGoogle Scholar
- Wolff JJ, Gu H, Gerig G, Elison JT, Styner M, Gouttard S. Differences in white matter fiber tract development present from 6 to 24 months in infants with autism. Am J Psychiatry. 2012;169:589–600.View ArticlePubMed CentralPubMedGoogle Scholar
- Jones W, Klin A. Attention to eyes is present but in decline in 2-6-month-old infants later diagnosed with autism. Nature. 2013;504:427.View ArticlePubMed CentralPubMedGoogle Scholar
- Sandin S, Lichtenstein P, Kuja-Halkola R, Larsson H, Hultman CM, Reichenberg A. The familial risk of autism. JAMA-J Am Med Assoc. 2014;311:1770–7.View ArticleGoogle Scholar
- Gaugler T, Klei L, Sanders SJ, Bodea CA, Goldberg AP, Lee AB, et al. Most genetic risk for autism resides with common variation. Nat Genet. 2014;46:881–5.View ArticlePubMed CentralPubMedGoogle Scholar
- Hallmayer J, Cleveland S, Torres A, Phillips J, Cohen B, Torigoe T, et al. Genetic heritability and shared environmental factors among twin pairs with autism. Arch Gen Psychiatry. 2011;68:1095–102.View ArticlePubMedGoogle Scholar
- Elsabbagh M, Fernandes J, Webb SJ, Dawson G, Charman T, Johnson MH. British autism study infant S: disengagement of visual attention in infancy is associated with emerging autism in toddlerhood. Biol Psychiatry. 2013;74:189–94.View ArticlePubMed CentralPubMedGoogle Scholar
- Ullian EM, McIntosh JM, Sargent PB. Rapid synaptic transmission in the avian ciliary ganglion is mediated by two distinct classes of nicotinic receptors. J Neurosci. 1997;17:7210–9.PubMedGoogle Scholar
- Gotti C, Zoli M, Clementi F. Brain nicotinic acetylcholine receptors: native subtypes and their relevance. Trends Pharmacol Sci. 2006;27:482–91.View ArticlePubMedGoogle Scholar
- Lavoie J, Maziade M, Hebert M. The brain through the retina: the flash electroretinogram as a tool to investigate psychiatric disorders. Prog Neuropsychopharmacol B ol Psychiatry. 2014;48:129–34.View ArticleGoogle Scholar
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.