Functionality of promoter microsatellites of arginine vasopressin receptor 1A (AVPR1A): implications for autism
© Tansey et al; licensee BioMed Central Ltd. 2011
Received: 7 September 2010
Accepted: 31 March 2011
Published: 31 March 2011
Arginine vasopressin (AVP) has been hypothesized to play a role in aetiology of autism based on a demonstrated involvement in the regulation of social behaviours. The arginine vasopressin receptor 1A gene (AVPR1A) is widely expressed in the brain and is considered to be a key receptor for regulation of social behaviour. Moreover, genetic variation at AVPR1A has been reported to be associated with autism. Evidence from non-human mammals implicates variation in the 5'-flanking region of AVPR1A in variable gene expression and social behaviour.
We examined four tagging single nucleotide polymorphisms (SNPs) (rs3803107, rs1042615, rs3741865, rs11174815) and three microsatellites (RS3, RS1 and AVR) at the AVPR1A gene for association in an autism cohort from Ireland. Two 5'-flanking region polymorphisms in the human AVPR1A, RS3 and RS1, were also tested for their effect on relative promoter activity.
The short alleles of RS1 and the SNP rs11174815 show weak association with autism in the Irish population (P = 0.036 and P = 0.008, respectively). Both RS1 and RS3 showed differences in relative promoter activity by length. Shorter repeat alleles of RS1 and RS3 decreased relative promoter activity in the human neuroblastoma cell line SH-SY5Y.
These aligning results can be interpreted as a functional route for this association, namely that shorter alleles of RS1 lead to decreased AVPR1A transcription, which may proffer increased susceptibility to the autism phenotype.
The neuropeptide arginine vasopressin (AVP) has been hypothesized to play a role in the aetiology of autism based on a demonstrated involvement in social bonding and in the regulation of a variety of socially relevant behaviours in animal models. Pharmacological experiments in rodents have shown a role for vasopressin in learning and memory, aggression and affiliative behaviours. Increases in AVP are associated with stressful or defensive circumstances and AVP is also involved in aggressive behaviours and pair bonding, mostly in males . AVP regulates male social behaviour not just through higher expression in males but also in steroid-sensitive brain sexual dimorphisms in AVP neurons [1, 2]. AVP may therefore influence sexually dimorphic social behaviours in a range of species . The role of sex hormones on AVP is of interest in the context of autism considering that the ratio of affected males with autism compared to affected females is highly skewed (4:1) . Only one study has examined the relationship between AVP levels and autism; Boso et al.  found AVP levels to be higher in plasma from individuals with autism than controls (P = 0.02).
AVP acts through binding to G-protein-coupled receptors. One of these receptors, arginine vasopressin receptor 1A (AVPR1A), is widely expressed in the brain. There is considerable evidence to implicate the AVPR1A gene in social behaviours. AVPR1A-knockout mouse models show impaired social recognition and decreased anxiety behaviours, while overexpression of AVPR1A in mice resulted in increased social memory [6, 7]. Disruption in AVPR1A expression in the brain has been associated with major behavioural changes [8–11]. In non-human mammals, the variable expression of AVPR1A is implicated in changes in social bonding, parental rearing behaviours and social recognition and memory. Hammock and Young  described behavioural differences in voles (genus Microtus) that was related back to the AVP neuropeptide. Prairie voles (Microtus ochrogaster) are socially monogamous through multiple breeding seasons with both the males and females contributing to rearing of the young. These behaviours are not typically seen within closely related species such as the montane vole (Microtus montanus) and meadow vole (Microtus pennsylvanicus). This phenomenon has been attributed to variable expression resulting from a microsatellite marker in the 5'-flanking region of the AVPR1A gene in Microtus [13, 14].
Various studies have linked different lengths of the AVPR1A polymorphisms to behavioural traits in non-clinical samples. Knafo et al.  reported that subjects with shorter length RS3 polymorphisms were found to be less generous by the 'dictator game' indicating less altruistic behaviour . Moreover, shorter variants in general were associated with lower levels of AVPR1A mRNA in postmortem hippocampus. Recent neuroimaging approaches have also indicated a link between gene expression levels and amygdala activation .
Recent studies have highlighted the role of rare de novo inherited copy number variants in the aetiology of autism . However, only rare de novo and inherited variants are established as genetic risk factors, and these account for a small proportion of the total genetic risk . Furthermore, in a recent paper from the International Schizophrenia Consortium, common genetic variants accounted for over 30% of the genetic risk for schizophrenia  and there is no reason to believe this will not be the case for autism as well. The genetic factors influencing the aetiology of autism are likely to be a combination of rare, private and more common variants. The action of which are not likely to be mutually exclusive.
Based on prior evidence suggesting a role for AVP in human and animal social cognition, and prior reports of genetic association between variants in vasopressin-related genes and autism, we hypothesized that variants in AVPR1A would be associated with autism in our Irish autism sample. We examined the variants RS1, RS3, AVR and four tagging single nucleotide polymorphisms (SNPs) across the gene locus, namely rs3803107, rs1042615, rs3741865 and rs11174815, for association with autism in the Irish autism trio collection. Furthermore, considering the evidence to support a role of 5'-flanking variation in the expression of AVPR1A in Microtus, we hypothesised that variation in the 5'-flanking region of the human AVPR1A gene at the microsatellites' RS3 and RS1 polymorphisms would influence the relative effects on promoter activity.
A total of 177 families, consisting of an affected child and both parents were recruited through schools, parent support groups and clinician referral. The male to female ratio was 4.68:1. Autism diagnoses were confirmed using Autism Diagnostic Interview - Revised (ADI-R)  and the Autism Diagnostic Observation Schedule - Generic (ADOS-G) . IQ and Vineland Adaptive Behaviour Score (VABS)  were evaluated to establish a distribution of functioning within the sample. Individuals with known medical causes of autism (for example, tuberous sclerosis, extreme prematurity, congenital rubella) were excluded. Subjects met the ADI-R criteria for autism, and the ADOS-G criteria for autism or ASD. All individuals had an IQ >35 or a mental age (as defined by the VABS) >18 months. Ethical approval was obtained from the Health Services Executive Linn Dara Child and Adolescent Psychiatry Ethics Committee, Ireland. DNA was extracted from blood or buccal swabs using the phenol/chloroform extraction method.
Using the HapMap CEPH data, four SNPs in the AVPR1A locus were identified using the tagger function of Haploview . SNPs were selected using pairwise tagging with an r2 threshold of 0.8 with an additional 2000 base pairs taken 5' and 3' of the gene transcript. SNP genotyping was performed by a commercial genotyping company, KBiosciences (http://www.kbioscience.co.uk/) using Kaspar assays. Three additional microsatellite polymorphisms (RS1, RS3, and AVR) at the AVPR1A gene locus were also genotyped. RS1: forward (fluorescent) 5'-AGG GAC TGG TTC TAC AAT CTG C-3'; reverse 5'-ACC TCT CAA GTT ATG TTG GTG G-3'. RS3: forward (fluorescent) 5'-CCT GTA GAG ATG TAA GTC CT-3'; reverse 5'-TCT GGA AGA GACT TAG ATG G-3'. AVR: forward (fluorescent) 5'-ATC CCA TGT CCG TCT GGA C-3'; reverse 5'-AGT GTT CCT CCA AGG TGC G-3' [15, 18]. Each reaction contained 0.5 mM primer. The sample was initially heated for 10 min at 95°C followed by 30 cycles of 95°C (30 s), 55°C (30 s), 72°C (40 s) and a final extension step of 72°C for 10 min. An ABI 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) was used to determine the length of each PCR product. Samples were analysed using Genemapper v2.7 (Applied Biosystems).
Construction of the reporter vectors
Cell culture and transfection
The human neuroblastoma cell line SH-SY5Y, were maintained in RPMI 1640 containing 10% foetal bovine serum (FBS) at 37°C and 5% CO2. Transfections were performed in triplicate a minimum of three times using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
Luciferase assays were performed using the dual-glow luciferase system (Promega) according to the manufactures' instructions. Luminescence was measured on a Victor plate reader (PerkinElmer, Boston, MA, USA). Transfection efficiency was corrected for by normalising data relative to the cotransfected renilla luciferase pRL-cytomegalovirus (CMV) control vector (Promega). For the RS3 and RS1 constructs, 100 ng of test constructs were cotransfected with 500 ng pGL3 control vector. Expression was reported as relative to the pGL3 control vector.
Transmission disequilibrium test (TDT) for the three microsatellites from AVPR1A were analysed in UNPHASED . Each allele is tested independently for transmission disequilibrium. Research previously undertaken in voles demonstrated the repeat length of the polymorphism modified gene expression, transcriptional pattern in the brain and social behaviours . Therefore, we decided to test for association between the length of polymorphic repeat and autism. Alleles were grouped in 'long' alleles and 'short' alleles for RS1, RS3 and AVR polymorphisms. Nomenclature of alleles for RS1 and RS3 can be found in Yirmiya et al. . For RS3, 'long' refers to allele 327 and all repeats longer than 327 and 'short' refers to allele 325 and all repeats shorter than allele 325 consistent with the previously published study of Knafo et al. . For RS1, 'long' consists of allele 314 and all repeats larger than allele 314 and 'short' consists of allele 310 and all repeats shorter than allele 310. For AVR, 'short' includes allele 213 and all repeats shorter than allele 213, while 'long' includes allele 215 and all repeats larger than allele 215. A similar procedure has been applied to other complex polymorphism, such as the nitric oxide synthase 1 (NOS1) ex1f- variable number tandem repeat (VNTR) [30–33].
Hardy-Weinberg equilibrium and TDT analyses for SNP markers were performed using PLINK . TDT for dichotomised microsatellites (long versus short) was also performed using PLINK. No Mendelian incompatibilities were detected and all variants were found to be in Hardy-Weinberg equilibrium (P > 0.01) in the parents. Permutation procedures were performed in PLINK using the mperm function (n = 1000). Haplotypes were defined using Gabriel's confidence intervals and tested for association using permutation in Haploview (n = 1000) [28, 35].
The statistical significance of differences in relative gene reporter activity was examined using two-tailed Student's t test in Stata v. 10 (StataCorp, College Station, TX, USA).
In the Irish autism sample (n = 177) at minor allele frequencies of 0.20, there was greater than 80% power to observe an effect of odds ratio (OR) approximately 1.35 at an uncorrected P = 0.05 level. This calculation considers a modest effect size and also no linkage disequilibrium between the test and causative marker.
Genetic association analyses
Genotype association results using UNPHASED for multiallelic markers and PLINK for dichotomous markers
0.008 a 0
Multimarker transmission disequilibrium test (TDT) for the microsatellite markers RS3, RS1 and AVR reporting the likelihood ratio statistic (LRS), degrees of freedom (df) and global P value from UNPHASED
Gene reporter assays for the RS1 and RS3 polymorphism of AVPR1A
In the current work we present the results of genetic investigations of the AVPR1A gene in an Irish autism sample. We followed association analysis with investigations to examine the role of promoter region polymorphisms, RS1 and RS3, on relative promoter activity.
Overall, we observed a single association with AVPR1A in the Irish autism trio collection (rs11174815, corrected P = 0.01). Due to the very low minor allele frequency (minor allele frequency (MAF) = 0.015) and lack of observations for transmissions of the A allele, it is difficult to conclude the relationship between this marker and autism in the Irish autism sample from our study. Furthermore, a weak association signal was seen between the dichotomised RS1 polymorphism and individuals with autism in the Irish sample. The short alleles were overtransmitted in individuals with autism.
We performed an experimental examination of the effect of allele length of the RS1 and RS3 polymorphism on relative promoter activity in the luciferase gene reporter assay. For the RS1 variant we observed the shorter allele to show lower relative promoter activity. Previous studies have reported shorter RS1 polymorphisms show more activity in the left amygdala in a non-clinical sample . Excessive amygdala activation during social interaction has been reported to be associated with increase anxiety and eventually social withdrawal . These aligning results suggest shorter alleles of RS1 result in lower relative promoter activity and possibly reduced transcription of AVPR1A, increasing amygdala activity leading to social withdrawal.
Results from previous studies on the arginine vasopressin receptor 1A gene (AVPR1A) RS3 polymorphism demonstrating the functionality of this region
In a recent study, the human RS1 polymorphism showed no differences in COS7 cells . Furthermore, Hong et al.  repeated the experiment in the PC12 cell line with no difference shown; however expression levels were extremely low indicating a potential weak promoter in AVPR1A. The difference in results between this study and ours is not surprising given recent evidence that cis-acting variation differs between tissue and cell types [40–44]. Our study examines the enhancer effects of RS1 and RS3 in an appropriate and relevant cell type for consideration in the aetiology of autism, a neurological disorder. In the study undertaken here, RS1 and RS3 are examined as enhancers modulating a viral promoter SV40 instead of its own natural promoter. Gene reporter systems do not take into account the genomic or chromatin context of the elements tested, however it is encouraging that our data agree with postmortem mRNA levels as shown in Knafo et al. , although additional studies are warranted. While the genomic environment tested was an artificial construction without the use of the endogenous promoter, these data do indicate that the repeat elements influence the relative activity of a core promoter in neuronal cells.
The results from both RS3 and RS1 luciferase gene reporter assays are intriguing, with potentially shorter variants of both RS3 and RS1 leading to decreases in AVPR1A mRNA. Our results align with the study undertaken on the Microtus microsatellites, which showed decreased microsatellite length contributes to decreased sociability . This suggests a potential conservation of function across species highlighting the importance of these polymorphisms. However, the functionality of both polymorphisms in our study complicates any conclusions. A limitation of the gene reporter approach is the removal of additional regulatory mechanisms, which may affect the DNA region of interest, and in these particular polymorphisms, how these polymorphisms may interact with each other in the regulation of AVPR1A transcription. Our haplotype analysis between the dichotomised alleles of RS1 and RS3 showed no association in the Irish sample. Rigorous meta-analysis is currently not possible due to the use of different genotyping methodologies . We have used the same genotyping primers as the Knafo et al. , Meyer-Lindenberg et al.  and Levin et al.  allowing us to draw conclusions easily across these studies. Future work examining the regulatory relationship between these two polymorphisms will be essential for better understanding of how they influence in vivo transcription of AVPR1A and ultimately how they potentially play a role in the aetiology of autism.
We thank the families who took part in this study for their support and cooperation. Family collection in Ireland and data analysis was supported by grants from the Health Research Board, Ireland, The Wellcome Trust, UK, and the National Alliance for Autism Research, USA.
- Goodson JL, Bass AH: Social behavior functions and related anatomical characteristics of vasotocin/vasopressin systems in vertebrates. Brain Res Brain Res Rev. 2001, 35: 246-265. 10.1016/S0165-0173(01)00043-1.View ArticlePubMedGoogle Scholar
- Bales KL, Plotsky PM, Young LJ, Lim MM, Grotte N, Ferrer E, Carter CS: Neonatal oxytocin manipulations have long-lasting, sexually dimorphic effects on vasopressin receptors. Neuroscience. 2007, 144: 38-45. 10.1016/j.neuroscience.2006.09.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Ferris WF, Naran NH, Crowther NJ, Rheeder P, van der Merwe L, Chetty N: The relationship between insulin sensitivity and serum adiponectin levels in three population groups. Horm Metab Res. 2005, 37: 695-701. 10.1055/s-2005-870580.View ArticlePubMedGoogle Scholar
- Chakrabarti S, Fombonne E: Pervasive developmental disorders in preschool children: confirmation of high prevalence. Am J Psychiatry. 2005, 162: 1133-1141. 10.1176/appi.ajp.162.6.1133.View ArticlePubMedGoogle Scholar
- Boso M, Emanuele E, Politi P, Pace A, Arra M, Ucelli di Nemi S, Barale F: Reduced plasma apelin levels in patients with autistic spectrum disorder. Arch Med Res. 2007, 38: 70-74. 10.1016/j.arcmed.2006.08.003.View ArticlePubMedGoogle Scholar
- Bielsky IF, Hu SB, Young LJ: Sexual dimorphism in the vasopressin system: lack of an altered behavioral phenotype in female V1a receptor knockout mice. Behav Brain Res. 2005, 164: 132-136. 10.1016/j.bbr.2005.06.005.View ArticlePubMedGoogle Scholar
- Bielsky IF, Hu SB, Szegda KL, Westphal H, Young LJ: Profound impairment in social recognition and reduction in anxiety-like behavior in vasopressin V1a receptor knockout mice. Neuropsychopharmacology. 2004, 29: 483-493. 10.1038/sj.npp.1300360.View ArticlePubMedGoogle Scholar
- Lim MM, Bielsky IF, Young LJ: Neuropeptides and the social brain: potential rodent models of autism. Int J Dev Neurosci. 2005, 23: 235-243. 10.1016/j.ijdevneu.2004.05.006.View ArticlePubMedGoogle Scholar
- Young LJ, Toloczko D, Insel TR: Localization of vasopressin (V1a) receptor binding and mRNA in the rhesus monkey brain. J Neuroendocrinol. 1999, 11: 291-297. 10.1046/j.1365-2826.1999.00332.x.View ArticlePubMedGoogle Scholar
- Insel TR, Wang ZX, Ferris CF: Patterns of brain vasopressin receptor distribution associated with social organization in microtine rodents. J Neurosci. 1994, 14: 5381-5392.PubMedGoogle Scholar
- Young LJ, Winslow JT, Nilsen R, Insel TR: Species differences in V1a receptor gene expression in monogamous and nonmonogamous voles: behavioral consequences. Behav Neurosci. 1997, 111: 599-605. 10.1037/0735-7044.111.3.599.View ArticlePubMedGoogle Scholar
- Hammock EA, Young LJ: Variation in the vasopressin V1a receptor promoter and expression: implications for inter- and intraspecific variation in social behaviour. Eur J Neurosci. 2002, 16: 399-402. 10.1046/j.1460-9568.2002.02083.x.View ArticlePubMedGoogle Scholar
- Hammock EA, Lim MM, Nair HP, Young LJ: Association of vasopressin 1a receptor levels with a regulatory microsatellite and behavior. Genes Brain Behav. 2005, 4: 289-301. 10.1111/j.1601-183X.2005.00119.x.View ArticlePubMedGoogle Scholar
- Hammock EA, Young LJ: Microsatellite instability generates diversity in brain and sociobehavioral traits. Science. 2005, 308: 1630-1634. 10.1126/science.1111427.View ArticlePubMedGoogle Scholar
- Thibonnier M, Graves MK, Wagner MS, Chatelain N, Soubrier F, Corvol P, Willard HF, Jeunemaitre X: Study of V(1)-vascular vasopressin receptor gene microsatellite polymorphisms in human essential hypertension. J Mol Cell Cardiol. 2000, 32: 557-564. 10.1006/jmcc.2000.1108.View ArticlePubMedGoogle Scholar
- Kim SJ, Young LJ, Gonen D, Veenstra-VanderWeele J, Courchesne R, Courchesne E, Lord C, Leventhal BL, Cook EH, Insel TR: Transmission disequilibrium testing of arginine vasopressin receptor 1A (AVPR1A) polymorphisms in autism. Mol Psychiatry. 2002, 7: 503-507. 10.1038/sj.mp.4001125.View ArticlePubMedGoogle Scholar
- Wassink TH, Piven J, Vieland VJ, Pietila J, Goedken RJ, Folstein SE, Sheffield VC: Examination of AVPR1a as an autism susceptibility gene. Mol Psychiatry. 2004, 9: 968-972. 10.1038/sj.mp.4001503.View ArticlePubMedGoogle Scholar
- Yirmiya N, Rosenberg C, Levi S, Salomon S, Shulman C, Nemanov L, Dina C, Ebstein RP: Association between the arginine vasopressin 1a receptor (AVPR1a) gene and autism in a family-based study: mediation by socialization skills. Mol Psychiatry. 2006, 11: 488-494. 10.1038/sj.mp.4001812.View ArticlePubMedGoogle Scholar
- Yang SY, Cho SC, Yoo HJ, Cho IH, Park M, Yoe J, Kim SA: Family-based association study of microsatellites in the 5' flanking region of AVPR1A with autism spectrum disorder in the Korean population. Psychiatry Res. 2010, 178: 199-201. 10.1016/j.psychres.2009.11.007.View ArticlePubMedGoogle Scholar
- Knafo A, Israel S, Darvasi A, Bachner-Melman R, Uzefovsky F, Cohen L, Feldman E, Lerer E, Laiba E, Raz Y, Nemanov L, Gritsenko I, Dina C, Agam G, Dean B, Bornstein G, Ebstein RP: Individual differences in allocation of funds in the dictator game associated with length of the arginine vasopressin 1a receptor RS3 promoter region and correlation between RS3 length and hippocampal mRNA. Genes Brain Behav. 2008, 7: 266-275. 10.1111/j.1601-183X.2007.00341.x.View ArticlePubMedGoogle Scholar
- Meyer-Lindenberg A, Kolachana B, Gold B, Olsh A, Nicodemus KK, Mattay V, Dean M, Weinberger DR: Genetic variants in AVPR1A linked to autism predict amygdala activation and personality traits in healthy humans. Mol Psychiatry. 2009, 14: 968-975. 10.1038/mp.2008.54.PubMed CentralView ArticlePubMedGoogle Scholar
- Pinto D, Pagnamenta AT, Klei L, Anney R, Merico D, Regan R, Conroy J, Magalhaes TR, Correia C, Abrahams BS, Almeida J, Bacchelli E, Bader GD, Bailey AJ, Baird G, Battaglia A, Berney T, Bolshakova N, Bölte S, Bolton PF, Bourgeron T, Brennan S, Brian J, Bryson SE, Carson AR, Casallo G, Casey J, Chung BH, Cochrane L, Corsello C: Functional impact of global rare copy number variation in autism spectrum disorders. Nature. 466: 368-372. 10.1038/nature09146.
- Anney R, Klei L, Pinto D, Regan R, Conroy J, Magalhaes TR, Correia C, Abrahams BS, Sykes N, Pagnamenta AT, Almeida J, Bacchelli E, Bailey AJ, Baird G, Battaglia A, Berney T, Bolshakova N, Bölte S, Bolton PF, Bourgeron T, Brennan S, Brian J, Carson AR, Casallo G, Casey J, Chu SH, Cochrane L, Corsello C, Crawford EL, Crossett A: A genome-wide scan for common alleles affecting risk for autism. Hum Mol Genet. 19: 4072-4082. 10.1093/hmg/ddq307.
- Purcell SM, Wray NR, Stone JL, Visscher PM, O'Donovan MC, Sullivan PF, Sklar P: Common polygenic variation contributes to risk of schizophrenia and bipolar disorder. Nature. 2009, 460: 748-752.PubMedGoogle Scholar
- Lord C, Rutter M, Le Couteur A: Autism Diagnostic Interview-Revised: a revised version of a diagnostic interview for caregivers of individuals with possible pervasive developmental disorders. J Autism Dev Disord. 1994, 24: 659-685. 10.1007/BF02172145.View ArticlePubMedGoogle Scholar
- Lord C, Risi S, Lambrecht L, Cook EH, Leventhal BL, DiLavore PC, Pickles A, Rutter M: The autism diagnostic observation schedule-generic: a standard measure of social and communication deficits associated with the spectrum of autism. J Autism Dev Disord. 2000, 30: 205-223. 10.1023/A:1005592401947.View ArticlePubMedGoogle Scholar
- Sparrow SS, Cicchetti DV: Diagnostic uses of the Vineland Adaptive Behavior Scales. J Pediatr Psychol. 1985, 10: 215-225. 10.1093/jpepsy/10.2.215.View ArticlePubMedGoogle Scholar
- Barrett JC, Fry B, Maller J, Daly MJ: Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics. 2005, 21: 263-265. 10.1093/bioinformatics/bth457.View ArticlePubMedGoogle Scholar
- Dudbridge F: Likelihood-based association analysis for nuclear families and unrelated subjects with missing genotype data. Hum Hered. 2008, 66: 87-98. 10.1159/000119108.PubMed CentralView ArticlePubMedGoogle Scholar
- Galimberti D, Scarpini E, Venturelli E, Strobel A, Herterich S, Fenoglio C, Guidi I, Scalabrini D, Cortini F, Bresolin N, Lesch KP, Reif A: Association of a NOS1 promoter repeat with Alzheimer's disease. Neurobiol Aging. 2008, 29: 1359-1365. 10.1016/j.neurobiolaging.2007.03.003.View ArticlePubMedGoogle Scholar
- Reif A, Herterich S, Strobel A, Ehlis AC, Saur D, Jacob CP, Wienker T, Topner T, Fritzen S, Walter U, Schmitt A, Fallgatter AJ, Lesch KP: A neuronal nitric oxide synthase (NOS-I) haplotype associated with schizophrenia modifies prefrontal cortex function. Mol Psychiatry. 2006, 11: 286-300. 10.1038/sj.mp.4001779.View ArticlePubMedGoogle Scholar
- Reif A, Jacob CP, Rujescu D, Herterich S, Lang S, Gutknecht L, Baehne CG, Strobel A, Freitag CM, Giegling I, Romanos M, Hartmann A, Rösler M, Renner TJ, Fallgatter AJ, Retz W, Ehlis AC, Lesch KP: Influence of functional variant of neuronal nitric oxide synthase on impulsive behaviors in humans. Arch Gen Psychiatry. 2009, 66: 41-50. 10.1001/archgenpsychiatry.2008.510.View ArticlePubMedGoogle Scholar
- Reif A, Kiive E, Kurrikoff T, Paaver M, Herterich S, Konstabel K, Tulviste T, Lesch KP, Harro J: A functional NOS1 promoter polymorphism interacts with adverse environment on functional and dysfunctional impulsivity. Psychopharmacology (Berl). 2010Google Scholar
- Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, Bender D, Maller J, Sklar P, de Bakker PI, Daly MJ, Sham PC: PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet. 2007, 81: 559-575. 10.1086/519795.PubMed CentralView ArticlePubMedGoogle Scholar
- Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, Blumenstiel B, Higgins J, DeFelice M, Lochner A, Faggart M, Liu-Cordero SN, Rotimi C, Adeyemo A, Cooper R, Ward R, Lander ES, Daly MJ, Altshuler D: The structure of haplotype blocks in the human genome. Science. 2002, 296: 2225-2229. 10.1126/science.1069424.View ArticlePubMedGoogle Scholar
- Meyer-Lindenberg A, Kolachana B, Gold B, Olsh A, Nicodemus KK, Mattay V, Dean M, Weinberger DR: Genetic variants in AVPR1A linked to autism predict amygdala activation and personality traits in healthy humans. Mol Psychiatry. 2009, 14: 968-75. 10.1038/mp.2008.54.PubMed CentralView ArticlePubMedGoogle Scholar
- Stein MB, Goldin PR, Sareen J, Zorrilla LT, Brown GG: Increased amygdala activation to angry and contemptuous faces in generalized social phobia. Arch Gen Psychiatry. 2002, 59: 1027-1034. 10.1001/archpsyc.59.11.1027.View ArticlePubMedGoogle Scholar
- Israel S, Lerer E, Shalev I, Uzefovsky F, Reibold M, Bachner-Melman R, Granot R, Bornstein G, Knafo A, Yirmiya N, Ebstein RP: Molecular genetic studies of the arginine vasopressin 1a receptor (AVPR1a) and the oxytocin receptor (OXTR) in human behaviour: from autism to altruism with some notes in between. Prog Brain Res. 2008, 170: 435-449. full_text.View ArticlePubMedGoogle Scholar
- Hong KW, Matsukawa R, Hirata Y, Hayasaka I, Murayama Y, Ito S, Inoue-Murayama M: Allele distribution and effect on reporter gene expression of vasopressin receptor gene (AVPR1a)-linked VNTR in primates. J Neural Transm. 2009, 116: 535-538. 10.1007/s00702-009-0219-8.View ArticlePubMedGoogle Scholar
- Cowles CR, Hirschhorn JN, Altshuler D, Lander ES: Detection of regulatory variation in mouse genes. Nat Genet. 2002, 32: 432-437. 10.1038/ng992.View ArticlePubMedGoogle Scholar
- Koch O, Kwiatkowski DP, Udalova IA: Context-specific functional effects of IFNGR1 promoter polymorphism. Hum Mol Genet. 2006, 15: 1475-1481. 10.1093/hmg/ddl071.View ArticlePubMedGoogle Scholar
- Sun C, Southard C, Witonsky DB, Olopade OI, Di Rienzo A: Allelic imbalance (AI) identifies novel tissue-specific cis-regulatory variation for human UGT2B15. Hum Mutat. 31: 99-107. 10.1002/humu.21145.
- Wilkins JM, Southam L, Price AJ, Mustafa Z, Carr A, Loughlin J: Extreme context specificity in differential allelic expression. Hum Mol Genet. 2007, 16: 537-546. 10.1093/hmg/ddl488.View ArticlePubMedGoogle Scholar
- Zhang K, Li JB, Gao Y, Egli D, Xie B, Deng J, Li Z, Lee JH, Aach J, Leproust EM, Eggan K, Church GM: Digital RNA allelotyping reveals tissue-specific and allele-specific gene expression in human. Nat Methods. 2009, 6: 613-618. 10.1038/nmeth.1357.PubMed CentralView ArticlePubMedGoogle Scholar
- Levin R, Heresco-Levy U, Bachner-Melman R, Israel S, Shalev I, Ebstein RP: Association between arginine vasopressin 1a receptor (AVPR1a) promoter region polymorphisms and prepulse inhibition. Psychoneuroendocrinology. 2009, 34: 901-908. 10.1016/j.psyneuen.2008.12.014.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.